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Literature summary extracted from

  • Metzger, L.E.; Raetz, C.R.
    Purification and characterization of the lipid A disaccharide synthase (LpxB) from Escherichia coli, a peripheral membrane protein (2009), Biochemistry, 48, 11559-11571.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.182 Triton X-100 between 0 and 0.5 mM Triton X-100 increases the apparent specific activity 5fold Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.182 expressed in Escherichia coli strain XL1-Blue Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.182 D89A mutant shows no residual catalytic activity Escherichia coli
2.4.1.182 R201A mutant shows no residual catalytic activity Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.182 membrane LpxB activity is dependent upon the bulk surface concentration of ist substrates in a mixed micelle assay system, suggesting that catalysis occurs at the membrane interface Escherichia coli 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.182 42000
-
 gel filtration Haemophilus influenzae
2.4.1.182 43000
-
 8 * 43000, SDS-PAGE Escherichia coli
2.4.1.182 360000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.182 Escherichia coli
-
-
-
2.4.1.182 Escherichia coli C41(DE3)
-
-
-
2.4.1.182 Haemophilus influenzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.182 Ni-NTA column chromatography and Superdex 200 gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.182 45
-
 enzyme from membrane-free lysate, at 30°C Escherichia coli
2.4.1.182 183
-
 after 4.0fold purification, at 30°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.182 UDP-2,3-diacylglucosamine + 2,3-diacylglucosamine 1-phosphate
-
 Haemophilus influenzae 2',3'-diacylglucosamine-(beta,1'-6)-2,3-diacylglucosamine 1-phosphate + UDP
-
 ?
2.4.1.182 UDP-2,3-diacylglucosamine + 2,3-diacylglucosamine 1-phosphate
-
 Escherichia coli 2',3'-diacylglucosamine-(beta,1'-6)-2,3-diacylglucosamine 1-phosphate + UDP
-
 ?
2.4.1.182 UDP-2,3-diacylglucosamine + 2,3-diacylglucosamine 1-phosphate
-
 Escherichia coli C41(DE3) 2',3'-diacylglucosamine-(beta,1'-6)-2,3-diacylglucosamine 1-phosphate + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.182 homooctamer 8 * 43000, SDS-PAGE Escherichia coli
2.4.1.182 monomer 1 * 42000, SDS-PAGE Haemophilus influenzae

Synonyms

EC Number Synonyms Comment Organism
2.4.1.182 lipid A disaccharide synthase
-
 Haemophilus influenzae
2.4.1.182 lipid A disaccharide synthase
-
 Escherichia coli
2.4.1.182 LpxB
-
 Haemophilus influenzae
2.4.1.182 LpxB
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.182 7.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.182 6 9
-
 Escherichia coli

General Information

EC Number General Information Comment Organism
2.4.1.182 metabolism catalyzes the fifth step of lipid A biosynthesis Haemophilus influenzae
2.4.1.182 metabolism catalyzes the fifth step of lipid A biosynthesis Escherichia coli